first you would need to load the file to a ftp site (with the same name everyday)... within eloqua you have to set up an auto synch to grab that file everyday... within the auto synch, make sure you add the contacts to a contact group/shared list and to the program that is opting them out.. within the program you can unsubscribe them, add to a different contact group/shared list, remove them from a particular group/list etc..
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